Sanyo Welder MCO-19M(UVH) User Manual

MCO-19M(UVH)MCO-19M(UV) MCO-19M Multi Gas Incubator INSTRUCTION MANUAL

9 INCUBATOR COMPONENTS 18 Rear Right Side 21Rear Left Side For MCO-19M(UVH)/19M(UV) or when MCO-

10 1. Outer door: The outer door is held to the frame with a magnetic seal. A door heater is installed in the door panel. The door opening is rev

11 23. Connecting port A/B for N2/O2 gas pipe: Refer to page 17 for gas cylinder connection. Ensure that the gas pressure is set at 0.05 MPaG (0.

12 Control panel and keypad 1. Upper limit regulator: This regulator is used to set the upper temperature limit. 2. OVERH

13 Remote alarm terminal The remote alarm terminal is located at the rear right side of the incubator. The alarm is outputted f

14 INSTALLATION SITE For correct operation of the Incubator, install it in a location with the following conditions. WARNING When using CO2 gas for

15 INSTALLATION 1. Remove the packing tape and clean up. Remove all the tape that is securing the doors and inner attachments. Open the doors for

16 Connection of CO2 gas cylinder WARNING When connecting a gas cylinder to the Incubator, confirm the gas type. Confirm that the connections

17 Connection of N2 (or O2 ) gas cylinder WARNING Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are

18 Connection of gas injection nozzle In the case of control of O2 density in the chamber, connect the gas injection nozzle to the gas injectio

1 CONTENTS INTRODUCTION P. 3 PRECAUTIONS FOR SAFE OPERATION P. 4 LABELS ON THE INCUBATOR P. 8 ENVIRONMENTAL CONDITIONS P.

19 PREVENTING CONTAMINATION To prevent contamination of the chamber, select a suitable installation site. z Avoid locations with high temperature

20 PRECAUTIONS FOR CULTURES z Leave space between culture containers. Always leave space for ventilation between culture containers (Petri dishes,

21 Using the Unlock Key z Unlocking when power is interrupted If power is interrupted to the MCO-19M(UVH)/19M(UV) with an MCO-HL installed,

22 LCD PANEL The following display (called the Top Display) will appear when the power switch is turned ON. The default temperature is 37.0oC, the de

23 9. Status display field Various status or alarms are displayed. ・When UV lamp is lit: “UV” is displayed in reverse video. (If the MCO-19M(UVH)/19

24 BASIC OPERATIONS ON CONTROL PANEL The following operation are possible through control panel: z Setting the temperature・・・The chamber temperature

25 BASIC PARAMETERS Setting the chamber temperature, CO2 and O2 density The setting procedure for the chamber temperature, CO2 and O2 density are

26 Setting the key lock 1. To set the key lock, change the value of the key lock parameter from 0 to 1 on the Stand-by Setting Display and press

27 Releasing the key lock 1. To remove the key lock, change the value of the key lock parameter from 1 to 0 on the Stand-by Setting Display and p

28 Setting the key lock password 1. From the Top Display, press the MENU Key to display the menu, select Tools, and press the ENTER Key.

2 CONTENTS SPECIFICATIONS P. 74 PERFORMANCE P. 75 SAFETY CHECK SHEET P. 76

29 4. Enter the new user password (4 digits), select OK, and press the ENTER Key. 5. Enter the new user password again, select OK, and p

30 Automatic N2 (or O2) gas cylinder changeover This incubator switches the gas supply lines when one N2 (or O2) gas cylinder becomes empty. N

31 6. When gas cylinder A is switched to B, remove gas cylinder A and replace it with new one. Note: Be careful when handling the empty N2 (or O2

32 Setting the upper limit alarm temperature An upper limit temperature alarm is provided with the Incubator. The alarm temperature can be chan

33 ALARM PARAMETERS 1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER Key. 2. Select Alarm

34 UV LAMP PARAMETERS A UV lamp is used with the MCO-19M(UVH)/MCO-19M(UV) or the MCO-19M with an MCO-19UVS UV Lamp Expansion Kit installed. Use the f

35 Precautions when using the UV lamp WARNING Do not look directly at UV light. UV light is harmful to the eyes. z Always use the humidifying pa

36 Setting the UV lamp ON period Use the following procedure to change the setting of the UV lamp ON period. 1. Press the MENU Key from the To

37 • The UV timer can be set from 0 to 30 min. The default setting is for 5 min. When the UV timer is set to zero, the UV lamp will not light. • If

38 OTHER PARAMETERS Setting the date, time, and log interval 1. Press the MENU Key from the Top Display to display the menu, select Tools and pr

3 INTRODUCTION Read this manual carefully before using the Product and follow the instructions for safety operation. Sanyo disavows any respon

39 3. The Date Time Display will appear. Set the date, time and log interval. ・Entering the date Example for October 1, 2010: Enter 101

40 Initial settings (LCD/DAQ parameters) 1. Select LCD/DAQ Setting from the Select Tools Display, press the MENU Key to display the menu, select

41 DISPLAYING THE LOG A log of the past chamber temperatures, CO2 densities and O2 densities can be displayed on a graph. 1. Press the MENU Key fr

42 2. The log will be displayed with dots. Press the Up Cursor Key and Down Cursor Key to switch between the temperature, CO2 density and O2 density

43 Transferring data Use the following procedure to transfer the log data to a PC. 1. To transfer the log data for one day, press the MENU Key

44 WATER LEVEL SENSOR This Incubator is equipped with a water level sensor for the humidifying pan. The sensor is set automatically when the humidi

45 ROUTINE MAINTENANCE Cleaning the chamber and inner attachments When using the Incubator for the first time or when performing H2O2 deconta

46 5. Pull out the N2/O2 gas injection nozzle and N2/O2 gas injection nozzle tube. (See Fig. 4.) 6. Pull out the humidifying pan. (See Fig. 5.)

47 12. Clean all the attachments with a diluted neutral detergent, and then rinse them thoroughly with distilled water. 13. Wipe the trays, the i

48 Filling the humidifying pan Use the following procedure to fill the humidifying pan or to replace the water. 1. Lift the humidifying pan c

4 PRECAUTIONS FOR SAFE OPERATION It is imperative that the user complies with this manual as it contains important safety advice. Items and proced

49 H2O2 DECONTAMINATION When the chamber has been contaminated, or when cleaning the chamber prior to starting a culture, H2O2 decontamination can be

50 CAUTION Perform H2O2 decontamination with the chamber attachments arranged as specified by Sanyo. Arranging them in a different way may result in

51 H2O2 decontamination Use the following procedure to perform H2O2 decontamination using the H2O2 Generator (MCO-HP). 1. Take out all trays, tr

52 6. Press the H2O2 Key for 3 seconds. The system check will start. 7. If the system is normal, the following display will appear. (If

53 9. After completion of H2O2 mist generation, “UV Resolve” will flash at the top of the screen, and H2O2 gas resolution by UV light will start.

54 12. Dilute the remaining H2O2 reagent in the H2O2 Generator with a large volume of water and dispose of it. Rinse and wash the H2O2 Generator wit

55 ALARMS, SAFETY, AND SELF-DIAGNOSIS The Incubator supports the following alarms, safety functions, and self-diagnostic functions. Table 1: Alar

56 Alarm or safety function Conditions Display Buzzer Safety operationO2 sensor error The measured voltage for the O2 sensor is abnormal. Err19

57 Table 2: Alarm and Safety Functions for H2O2 Decontamination Alarm and safety function Condition Display Buzzer Safety operation System check

58 CALIBRATION Temperature/CO2/O2 calibration 1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTE

5 Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to rain water. Only qualified engineer

59 Example Enter the value shown below if the displayed chamber CO2 density is 5.0% but the actual CO2 density is 4.5%. ●CO2 density span: Enter

60 TROUBLESHOOTING If the Incubator does not seem to be working properly, check the following items before calling for service. Symptom Items t

61 Symptom Items to check and countermeasures A large quantity of gas is being consumed. • Are the doors being frequently opened and closed? •

62 DISPOSING OF THE MULTI-GAS INCUBATOR When disposing of the multi-gas incubator, contact a Sanyo sales representative or agent. WARNING The

63 Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC (English) This SANYO product is designed and manufactured

64 (French) Votre produit Sanyo est conçu et fabriqué avec des matèriels et des composants de qualité supérieure qui peuvent être re

65 (Portuguese) O seu produto SANYO foi concebido e produzido com materiais e componentes de alta qualidade que podem ser reciclados

66 (Dutch) Sanyo producten zijn ontwikkeld en gefabriceerd uit eerste kwaliteit materialen, de onderdelen kunnen worden gerecycled en

67 AUTOMATIC CO2 CYLINDER CHANGEOVER An automatic CO2 cylinder changeover system (MCO-21GC) is available as an optional accessory. This system swi

68 6. When gas cylinder A is switched to B, remove gas cylinder A and replace it with new one. Note: Be careful when handling the empty CO2 gas c

6 Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance. These may be harmful to your

69 AUTOMATIC CO2 AND O2 DENSITY CALIBRATION To calibrate the CO2 and O2 density, a Semi-automatic one point Gas Calibration Kit (MCO-SG) is available

70 3. Input the CO2 and O2 density of standard gas to be used, press the MENU Key to display the menu, select OK, and press the ENTER Key.

71 3. If the system is normal, the following display will appear. Select OK and press the ENTER Key to start calibrating the CO2 and O2 density. Aft

72 STACKING INCUBATORS Use the following procedure to stack Incubators. This work is potentially dangerous, so contact a Sanyo representative or a

73 Fig.AStacking plate B Stacking plate A Protective sticker Front panel Hook Front

74 SPECIFICATIONS Name Multi-gas Incubator Models MCO-19M MCO-19M(UV) MCO-19M(UVH) External dimensions W620 x D710 x H900 mm (W24.4 x D28.0 x H35

75 Name Multi-gas Incubator Models MCO-19M MCO-19M(UV) MCO-19M(UVH) Accessories 3 trays, 3 sets of tray supports, 3 gas tubes, 1 humidifying pan,

76 CAUTION Please fill in this form before servicing. Hand over this form to the service engineer to keep for his and your safety. Safety chec

7FB6P101569000 (26 August 2010) 5-5, Keihan-Hondori 2-Chome Moriguchi City, Osaka 570-8677 Japan Printed in Japan

7 Use a dedicated power source (a dedicated circuit with a breaker) as indicated on the rating label attached to the unit. A branched circu

8 LABELS ON THE INCUBATOR Warning and caution labels are attached to the Incubator. The following table describes the labels. This label is at